Small RNAs in the testis and ovary of mice before and after sexual maturation

For a considerable period, reproductive health, fertility, and reproductive-related diseases have posed challenges to human well-being, as well as to the conservation of endangered species and the advancement of animal husbandry. PANDORA-seq, a recently introduced sequencing technique, demonstrates heightened sensitivity towards highly modified small RNAs like tsRNA and rsRNA. In this research endeavor, we leveraged PANDORA-seq to capture the small RNA expression profiles of mouse testes and ovaries pre- and post-sexual maturation. Our investigation successfully pinpointed an array of abundantly expressed small RNAs across various tissues, encompassing tsRNA, rsRNA, piRNA, miRNA, snoRNA, and ysRNA. Furthermore, we conducted detailed expression feature analyses specifically focusing on tsRNA, rsRNA, piRNA, and miRNA. These datasets stand poised to serve as invaluable resources for delving into the postnatal development of testes and ovaries, shedding light on the epigenetic mechanisms underpinning germ cell production and differentiation. This study utilized 12 mice (C57BL/6J strain, purchased from CHENGDUDASHUO). The mice were evenly divided into four groups based on birth time and gender: 3-week-old male mice, 4-week-old female mice, 10-week-old male mice, and female mice.To investigate the distribution patterns and dynamic changes of sncRNAs in the testes and ovaries of mice before and after sexual maturation, we performed PANDORA-seq analysis and annotated small RNA sequences using SPORTS1.1 software on testicular and ovarian tissues collected from pre- and post-sexually matured C57/BL6 mice.