Transcriptome profiles of porcine oocytes with different developmental competence

the objectives of this study was to identify and analyze the transcriptome profiles of porcine oocytes aspirated from follicles of different sizes which showed differences in developmental competence and chromatin configurations using RNA high throughput sequencing technology Overall design: Cumulus-oocyte complexes (COCs) were aspirated from large (LO; 3-6 mm) or small (SO; 1.5-1.9 mm) ovarian follicles and tested for developmental competence and chromatin configurations. RNA libraries were constructed from both oocyte groups and then sequenced on an Illumina HiSeq4000