Plasma miRNA Differential Expression Analysis and miRNA-mRNA Network Construction in Patients with Acute Pancreatitis

In the early stages of acute pancreatitis, diagnostic sensitivity is limited and prognosis is difficult to determine. We provide a new idea for the diagnosis and prognosis of AP by studying the expression of plasma miRNAs in patients. Plasma samples from 22 normal subjects and AP patients at different stages were screened for miRNAs sequencing. A total of 27 miRNAs were found to be differentially expressed. Then we performed downstream target gene prediction for differentially expressed miRNAs. Analysising of KEGG, GO, GSEA and network interaction, it was found that its therapeutic effect was closely related to apoptosis, inflammatory response and autophagy process. By setting strict inclusion and exclusion criteria, plasma of 6 normal subjects and 16 patients with AP at different stages were screened. After RNA extraction from plasma samples, miRNAs were sequenced for the samples to find the differentially expressed miRNAs in AP patients, and bioinformatics analysis of differentially expressed miRNAs was performed.