Richard Allen (University of Hawaii) (2011) CIL:9850, Paramecium tetraurelia, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

Immunogold labeled microtubules next to the cytoproct radiate from basal bodies and also from the tip of the cytoproct. These microtubules lie along the approaching spent DV. b-tubulin immunogold labeled TEM image taken on 7/5/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 12,000X. Bar = 0.5µm. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:1307) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

免疫金标记的微管从细胞质突的基体以及细胞质突的尖端辐射开来，这些微管沿着即将耗尽的DV（动静脉）排列。在1996年7月5日，由R. Allen使用80kV的Zeiss 10A显微镜拍摄到的b-微管免疫金标记的透射电子显微镜（TEM）图像。负像放大倍数为12,000倍。图像标尺为0.5微米。细胞经过0.25%戊二醛轻微固定，并使用2.3M蔗糖渗透，之后在液氮中速冻，并在-100°C的温度下切割成约75纳米厚度的薄片。从这些制备的冷冻切片中，随后进行解冻、清洗，并暴露于由小鼠或兔/山羊制备的单克隆一级抗体以及与胶体金复合的山羊抗小鼠/兔二级抗体。有关制备的更多细节详见《细胞生物学方法》2010年第96卷第143-173页。负片被打印到纸上，并扫描至Photoshop。此数字化图像可供进行定性分析。未经处理的、高分辨率的图像（CIL:1307）存档于图书馆，可供定量分析。更多信息请访问（http://www5.pbrc.hawaii.edu/allen/）。