mRNA sequencing on esophageal organoids derived from wild-type mice

In our study, we used mRNA sequencing to investigate the effect of 3 days-treatment with TGFb1, BMP4 alone or in combination on the squamous and columnar differentiation. Overall design: mRNA profiles of esophageal organoids derived from wild-type mice treated for 72h with TGFb inhibitor / BMP4 / TGFb / TGFb + BMP4 by deep sequencing using Illumina