Supplementary Material - A Thousand Meters Deep: Vertical Profiling of the Subterranean Microbiome of Gourgouthakas Cave

Here we deposited the supplementary material of our work on the microbial isolates from Gourgouthakas cave (-1100 m depth). We isolated a total of 820 isolates from nine solid samples from the rock surface as shown in Figure 1 (see also supplementary material). We obtained the highest number of isolates on R2A (363 isolates), followed by NA1/2 (218), NA (137), and MNFMM (101). The majority of isolates grew at 28 °C (574 isolates), while 236 isolates grew at 5 °C, and 10 isolates grew at 5 °C but also showed growth at 28 °C.