Research data supporting "High-throughput 3D phenotyping of plant shoot apical meristems from tissue-resolution data"

This directory contains all the original data from the publication "High-throughput 3D phenotyping of plant shoot apical meristems from tissue-resolution data". Title: Research data supporting High-throughput 3D phenotyping of plant shoot apical meristems from tissue-resolution data Authors: Henrik Åhl, Yi Zhang, Henrik Jönsson Contact: [e-mail] Date of data collection: 2017-2021 Associated DOI: 10.3389/fpls.2022.827147 Location: Sainsbury Laboratory, University of Cambridge Keywords: pin3, pin4, pin7, pin3pin4, pin3pin7, pin4pin7, pin3pin4pin7, aux1, aux1lax1lax2lax3, abcb1, abcb19, abcb1abcb19, pCLV3:dsRed x pPIN1:PIN1-GFP x myr-YFP, xxt1xxt2, xxt1xxt2xxt5, qua1, bag, cesa3(eli1), cesa6(prc1-1), csi1-3, det3, cesa1(any1), gabi, cu4, cmu1cmu2, cesa3(je5), act2-5, arabidopsis thaliana, shoot apical meristem, confocal, isosurface, phyllotaxis, apex identification, tissue segmentation, plant development, flower development, 3D phenotyping, high-throughput Microscopes: LSM880, LSM700, LSM780 Formats: .csv, .txt, .lsm, .tif This dataset contains 3D image stacks of the following: - Col-0 plant shoots - pin3, pin4, pin7, pin3,4, pin3,7, pin4,7, pin3,4,7 mutant plants - aux1, aux1lax1,2,3 mutant plant shoots - abcb1, abcb19, abcb1,19 mutant plant shoots - pCLV3::dsRed x pPIN1:PIN1-GFP x myr-YFP expressing plant shoots - xxt1xxt2, xxt1xxt2xxt5, qua1*, bag*, cesa3(eli1), cesa6(prc1-1), csi1-3*, det3*, cesa1(any1), gabi*, cu4*, cmu1cmu2*, cesa3(je5), act2-5* mutant plant shoots (*: not used in the analysis) - One pABCB:ABCB19-GFP expressing plant - One Col-0 protoplast - One Col-0 apical hook The dataset also contains the output results from the analysis pipeline presented in the affiliated publication, as well as corresponding file metadata. Code and scripts used for data generation and statistical analysis, including visualisation can be found at https://gitlab.com/slcu/teamHJ/publications/aahl_etal_2022.

搜集汇总

背景与挑战

背景概述

该数据集支持'High-throughput 3D phenotyping of plant shoot apical meristems from tissue-resolution data'研究，包含2017-2021年收集的多种拟南芥突变体和转基因植物的3D图像堆栈，使用LSM系列显微镜采集，格式为.csv、.txt、.lsm、.tif。数据集专注于植物茎尖分生组织的高通量3D表型分析，涵盖广泛突变体类型，并提供分析管道输出结果和元数据，适用于植物发育和表型研究。