Characterization of pancreatic progenitor differentiation with GiBi-generated DE cells

We report the GiBi protocol to generate functional DE cells that can further enter pancreatic lineages. To understand the transcriptome dynamics during the generation of these pancreatic progenitor cells, we harvested cells at different time points of the differentiation for RNA sequencing. We find that during this process, genes that are related to pluripotency maintenance were decreasing and gastrulation-related genes were transiently expressed in the middle. More importantly, genes that are critical in pancreatic specification got significantly upregulated across the differentiation. Overall design: Every other day from D0 to D14 of the pancreatic progenitor differentiation, two biological replicates for each time point, we harvested in total 14 samples for RNA sequencing.