Hybridization Capture Sequencing for Vibrio spp. and Associated Virulence Factors

In the study reported here, hybridization capture sequencing (HCS) was employed to profile low abundant Vibrio spp. in metagenomic samples. This approach was evaluated in parallel with traditional whole community shotgun sequencing in a metagenomic analysis of water and oyster samples collected from the Chesapeake Bay. In addition, V. parahaemolyticus and V. vulnificus strains isolated from the samples were subjected to whole genome sequencing to determine the genetic characteristics of pathogenic Vibrio spp. circulating in an aquatic environment.