Nuclear localization of fly Pcif1 is required for gene regulation and fertility

Here we show that Pcif1 mutant flies display a reduced fertility which is particularly marked in females. Deep sequencing analysis of Pcif1 mutant ovaries revealed transcriptome changes with a notable increase in expression of genes belonging to the mitochondrial ATP synthetase complex. The RNA-seq was used to analyze the gene expression changes in ovaries of Pcif1M6stop and Pcif1F9ΔNLS fly lines when compared to control Pcif1BG02557 fly line. Three biological replicas were analyzed for each line.