Transcriptomic analysis of isolated zebrafish podocytes and endothelial cells exposed to human APOL1 mRNA

We injected zebrafish embryos with human APOL1 mRNA, dissociated embryos at 4 days of age, and purified podocytes and endothelial cells with fluorescence activated cell-sorting. After sorting, mRNA was extracted from purified cells and used to generate cDNA libraries, which were sequenced on an Illumina HiSeq 2500. Examination of two cell types exposed to one of 3 mRNA treatments (sham, APOL1 G0, APOL1 G2)