Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors [iR_iHEC]

We inserted the inducible expression cassette of Runx1 into the Rosa26 locus of embryonic stem cells. Based on a modified protocol for HEC induction from PSC, we successfully generated iHEC Under the haematopoietic induction process (iR-HEC). Overall design: mRNA profiles of single hematopoietic endothelia cells (iR-iHEC). single iR-iHECs were sorted from PSC-derived cells for RNAseq analysis according to kit instruction of Discover-sc WTA Kit V2(Vazyme) and TruePrepTM DNA Library Prep Kit V2 (Vazyme), using illumina HiSeq X Ten.