Integrated multiomics profiling of grapevine in response to viral mixed infection

In a recent study, we revealed a diverse virome (all the viruses and viroids) of a leafroll-infected interspecific hybrid grapevine cultivar and compared it to the virome of V. vinifera cultivar. However, co-occurrence analysis revealed that the presence of grapevine leafroll-associated virus species was randomly associated with the development of virus-like symptoms. To understand what drives the expression of symptoms, we used dsRNA and total RNA sequencing analysis to profile the virome and transcriptome of grapevine leaves collected at two different time points during the growing season