BMS493 injection regimes.

The same BMS493 concentration (Conc BMS) was used across injection regimes, from I to VI. Pink cases mark injection time points in the morning (mo), midday (mid) or evening (eve) between E10.5 and E12.5. The number of embryos with different muscle phenotype severity at E13.5 are shown: (no), no altered phenotype; mild (+), muscle mispatterning but overall organization retained; strong (++), strong mispatterning but with a minimum of 2 muscles split; severe (+++), no or almost absent splitting. n is the total number of embryos analyzed for a certain treatment type (n = 38 embryos analyzed in total); N exp, number of times the experiment was repeated. E, embryonic day; EOM, extraocular muscle. (XLSX)