RNA sequencing comparison of B7-H3.TMIGD2 and B7-H3.CD28.4-1BB CAR-T cells in vivo.

The tumor microenvironment can significantly alter CAR-T cell phenotypes. We therefore performed RNA sequencing to understand the effect of TMIGD2 vs CD28.4-1BB comstimulation in B7-H3 CAR-T cells after intravenous administration to tumor-bearing mice in vivo. We injected CAR-T cells intravenously in HCC827 lung tumor-bearing-mice. We isolated lung-infiltrating T cells, isolated T cell RNA, and finally performed RNA sequencing.