Supplementary materials for the article reporting Pacific Oyster Nidovirus 1 (PONV1)

Here, we provide supplementary materials, including phylogenetic trees, sequences, and relevant codes, for the article (Zhong <i>et al.</i> 2025; https://doi.org/10.1073/pnas.2426923122) reporting Pacific Oyster Nidovirus 1 (PONV1), detected in farms where Pacific oysters experiencing mass mortality events in British Columbia, Canada.<br><b>Contents of the sequence file:</b><b>Rdrp_containing_contig_unique_vOTU_189.fasta</b><br>Representative nucleotide sequences of 189 RdRp-containing RNA virus operational taxonomic units (vOTUs) identified from the Pacific oyster samples (ENA accession numbers: ERR13334386 to ERR13334481).<b>PONV1_segment_1.fasta</b><br>Nucleotide sequences of segment 1 from PONV1.<br>NCBI accession number: PQ030832.<b>PONV1_segment_2.fasta</b><br>Nucleotide sequences of segment 2 from PONV1.<br>NCBI accession number: PV579160.<b>PONV1-like_virus_segment_1.fasta</b><br>Nucleotide sequences of segment 1 from PONV1-like virus 1 through 15.<br>NCBI accession numbers: BK068944 to BK068958.<b>PONV1-like_virus_segment_2.fasta</b><br>Nucleotide sequences of segment 2 from PONV1-like virus 1 through 15.<br>NCBI accession numbers: PV928314 to PV928328.<br><b>Reference:</b>Zhong KX, Chan AM, Miller KM, Saunders R, and Suttle CA (2025) Evolutionarily divergent nidovirus with an exceptionally large genome identified in Pacific oysters undergoing mass mortality. <i>Proceedings of the National Academy of Sciences USA</i>, <b>122:</b> e2426923122. https://doi.org/10.1073/pnas.2426923122<br><br><br>