Code for analysis of lysosome distance from nucleus

Cells were cultured in Ibidi 4-well µ-slides for three days. Prior to imaging, the medium was replaced with HEPES-buffered D5030 medium titrated to pH 7.4. Lysotracker Green DND 26 and Hoechst33342 were added to the medium at concentration of 100 nM and 10 µg/mL, respectively. After 10 min of incubation, Lysotracker fluorescence was recorded at 488 nm excitation, emission >510 nm using Zeiss LSM 700 confocal microscope.

细胞在Ibidi 4-well µ-slides中进行培养，持续三天。在成像之前，培养基被更换为pH值调至7.4的HEPES缓冲液D5030。将Lysotracker Green DND 26和Hoechst 33342分别以100 nM和10 µg/mL的浓度添加至培养基中。经过10分钟的孵育后，使用Zeiss LSM 700共聚焦显微镜，在488 nm激发光下记录Lysotracker的荧光，发射波长大于510 nm。