Distinct populations of crypt-associated fibroblasts act as signaling hubs to control colon homeostasis

Purpose: to determine cellular heterogeneity of the murine colonic epithelium and mesenchyme; Summary: We determined 16 disctinct sub-populations of colonic epithelial and mesenchymal cells. Further we found 3 distinct sub-populations of colonic PdgfraEGFP-positive fibroblasts. Overall design: Wildtype murine colon cells were sorted using FACS (using EpCAM and CD45 antibodies). PdgfraEGFP intestinal mesenchymal cells were sorted using FACS (GFP positvity). Single cell RNA sequencing was perfomed (for details see Brügger et al, in prep)