DR, imposed by the eat-2(ad465) mutation, extended adult lifespan of daf-2(e1370), but not daf-2(e1368).

Adult lifespan was examined at 20°C in the presence of FUDR. Data are cumulative of two trails with 30–40 animals/trial. Two additional trials were conducted for ad lib and DR daf-2(e1370) and wildtype animals with consistent results (not shown).*Non-DR lifespans of daf-2(e1370) versus daf-2(e1368) were statistically significantly different by the Log-rank test (p = 0.0058) but not by Wilcoxon test p = 0.11), although they were not dramatically different.