Genome annotations of five cephalochordate species

This data repository contains the gene annotation files that we generated for five cephalochordate species: Alu: Asymmetron lucayanum Bbe: Branchiostoma belcheri Bfl: Branchiostoma floridae Bja: Branchiostoma japonicum Bla: Branchiostoma lanceolatum The Alu genome was sequenced by our team (www.evomicslab.org) with collaboration with Dr. Jr-Kai Yu, Dr. Sung-Jin Cho, and Dr. Linda Holland. Two haplotype-decoupled assemblies were produced corresponding to the reference (filename: Asymmetron_lucayanum.ref.*) and alternative (filename: Asymmetron_lucayanum.alt.*). The assembly file of these two genome assemblies have been deposited in Genome Warehouse of National Genomics Data Center (https://ngdc.cncb.ac.cn/gwh) under the accession numbers of GWHFWAS00000000.1 and GWHFWAV00000000.1 respectively. The Bbe, Bfl, Bja, and Bla genome assemblies were generated by previous studies: Species NCBI Genbank accession number Reference Bbe GCA_019207075.1 Huang et al. (2023) Three amphioxus reference genomes reveal gene and chromosome evolution of chordates. PNAS, 120 (10), e2201504120 Bfl GCA_019207045.1 Huang et al. (2023) Three amphioxus reference genomes reveal gene and chromosome evolution of chordates. PNAS, 120 (10), e2201504120 Bja GCA_013266295.2 Huang et al. (2023) Three amphioxus reference genomes reveal gene and chromosome evolution of chordates. PNAS, 120 (10), e2201504120 Bla GCA_927797965.1 Brasó-Vives et al. (2022) Parallel evolution of amphioxus and vertebrate small-scale gene duplications. Genome Biology, 23 (1), 243 All 6 genome assemblies were annotated with the same annotation pipeline in our study: RepeatMasker (v4.1.1) and EDTA (v2.0.1) for repeats/TE annotation, FunAnnotate (v1.8.15) for protein coding gene and tRNA gene annotation, RNAmmer (v1.2) for rRNA gene annotation. Explanation for each files: Species_name.gff3.gz # protein-coding gene and tRNA gene annotation in GFF3 format (compressed by gzip) Species_name.cds-transcripts.fa.gz # CDS/transcript sequences of annotated protein-coding genes in FASTA format (compressed by gzip) Species_name.proteins.fa.gz # protein sequences of annotated protein-coding genes in FASTA format (compressed by gzip) Species_name.rRNA.gff2.gz # rRNA gene annotation in GFF2 format (compressed by gzip) Species_name.TEanno.gff3.gz # TE annotation in GFF3 format (compressed by gzip) In addition, we generated some potentially valuable intermediate and final result files for various comparative genomics analyses. AluAsRef_amphioxus_5way_WGA.maf.gz # The multi-way whole-genome alignment files across the five amphioxus species (Alu, Bbe, Bfl, Bja, Bla). The Alu reference genome was used as reference for providing genomic coordinates. HsaAsRef_vertebrate_6way_WGA.maf.gz # The multi-way whole-genome alignment files across the fix vertebrate species (spotted gar, frog, chicken, platypus, mouse, human). The human reference genome was used as reference for providing genomic coordinates. Alu_ref.Hsa.final.axt.gz # The pairwise whole-genome alignment file between the Alu and human. five_amphioxus_Hox_CDS_aln.tar.gz # The full-length CDS alignment for orthologous Hox genes across the five amphioxus species (Alu, Bbe, Bfl, Bja, Bla). human_chimpanzee_Hox_CDS_aln.tar.gz # The full-length CDS alignment for orthologous Hox genes between human and chimpanzee. Hox.protein.trimmed.fa # The trimmed full-length protein alignment for the Hox gene family. Hox.protein.trimmed.treefile # The trimmed full-length protein tree (newick format) for the Hox gene family. TLR.protein.trimmed.fa # The trimmed full-length protein alignment for the TLR gene family. TLR.protein.trimmed.treefile # The trimmed full-length protein tree (newick format) for the TLR gene family. NLR.protein.trimmed.fa # The trimmed full-length protein alignment for the NLR gene family. NLR.protein.trimmed.treefile # The trimmed full-length protein tree (newick format) for the NLR gene family. RAG1.protein.trimmed.fa # The trimmed full-length protein alignment for the RAG1(-like) gene family. RAG1.protein.trimmed.treefile # The trimmed full-length protein tree (newick format) for the RAG1(-like) gene family. RAG2.protein.trimmed.fa # The trimmed full-length protein alignment for the RAG2(-like) gene family. RAG2.protein.trimmed.treefile # The trimmed full-length protein tree (newick format) for the RAG2(-like) gene family. species_phylogeny.protein.trimmed.fa # The concatednated and trimmed protein alingment based on 947 one-to-one orthologous genes. species_phylogeny.protein.trimmed.treefile # The concatednated and trimmed protein tree based on 947 one-to-one orthologous genes. Finally, the supplementary dataset S1-S5 for this study: DatasetS1.New2old_gene_annotation_correspondence_table.xlsx # The comparison between our new gene annotation and the previously published gene annotation for the four Branchiostoma species: Bfl, Bla, Bja, Bbe. DatasetS2.Hox.protein.trimmed.tree.pdf # The trimmed full-length protein tree (printed version) for the Hox gene family. DatasetS3.TLR.protein.trimmed.tree.pdf # The trimmed full-length protein tree (printed version) for the TLR gene family. DatasetS4.NLR.protein.trimmed.tree.pdf # NLR.protein.trimmed.treefile # The trimmed full-length protein tree (printed version) for the NLR gene family. DatasetS5.cephalochordate_transcriptome_TPM.xlsx # Our curated the multi-stage bulk RNA-seq datasets (both from this study and published studies) of the five cephalochordate species used in this study.