Transcriptional activity and epigenetic regulation of transposable elements in the symbiotic fungus Rhizophagus irregularis

We report polyA+ mRNA profiles in a developmental assay where R. irregularis spores were exposed to either medium containing rice root-derived exudates or a mock nutrient medium. We report small RNA repertoires produced from R. irregularis spores using two different isolation techniques. We report the application of single-molecule-based DNA sequencing for profiling of CG methylation in untreated R. irregularis. spores. Overall design: Examination of mRNA profiles in mock and exudate-treated spores at three time points: a 0h untreated time point, 24h and 48h post treatment. RNAseq reads were generated by deep sequencing, in quadruplicate, using paired-end 150bp Illumina sequencing. Small RNA profiles were produced by oxidation (NaIO4 treatment) and Argonaute isolation (Lexogen TraPR column treatments), and compared to untreated controls.