SARS-CoV-2 Variant of Concern Fitness and Adaptation in Primary Human Airway Epithelia

Purpose: To identify the cell types change and gene expression change after infection with SARS-CoV-2 variants on primary human airway epithelial cells. Overall design: Methods: The primary human large/small airway epithelial cells (LAE/SAE) were cultured on air-liquid interface (ALI) conditions. After 4 weeks, the cells were infected with MOI 0.05 SARS-CoV-2. After 48-96 hours post infection, cells were dissociated with Accutase solution and proceeded to scRNA-seq. single cell-based mRNA profiles of SARS-CoV-2 D614G, Delta, or Omicron infected HAE cells