The effect of antibiotic induced imbalance of gut microbiota on the expression profile of miRNA in circulating extracellular vesicles

By measuring the miRNA expression profile in the blood circulating extracellular vesicles of mice with antibiotic induced intestinal microbiota imbalance through miRNA seq, it was determined that intestinal microbiota imbalance leads to significant changes in the miRNA expression profile in the blood circulating extracellular vesicles Overall design: Mice were given a mixture of antibiotics (Vancomycin 0.5 mg/ml, Neomycin 1 mg/ml, Ampicillin 1 mg/ml, Metronidazole 1 mg/ml; Shanghai yuanye Bio-Technology Co., Ltd) in drinking water for four weeks according to established protocols,isolate the serum exosomes by differential centrifugation.