Pro-BMP9 and sENG monomer:BMP9 complex signalling in human pulmonary arterial endothelial cells after 1.5 hours treatment
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE119206
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This experiment was desinged to investigate the difference in global gene expression induced by the circulating form of BMP9, pro-BMP9, and BMP9 in complex with soluble endoglin (sENG:BMP9). Four independent PAECs (i.e. isolated from four different individuals) were purchased from Lonza. Each line was grown up and seeded separately for the treatment by pro-BMP9 and sENG:BMP9, both at 0.4 ng/ml of final growth factor domain concentration. Treatment was carried out for 1.5 hours at 37 C to allow the detection of the genes directly regulated by BMP9 ligand complexes.
创建时间:
2019-09-23



