Defining splicing factor requirements for androgen receptor variant synthesis in advanced prostate cancer

To understand the function of splcing factor MFAP1 in the transcriptional regulation of anrogen receptor variants, we used the CWR22Rv1 prostate cancer cell line depleted of MFAP1 (siMFAP1) or AR-V7 (siCE3) for 72 hours. We then performed gene expression profiling analysis using data obtained from RNA-seq. Overall design: To investigate the AR-dependent and -independent global transcriptomic impact of MFAP1-depletion using CWR22Rv1 cells depleted of MFAP1 or AR-V7 using siRNA. We then performed gene expression profiling from the RNA sequencing data in response to MFAP1 or AR-V7 depletion compared to the non-targeting scrambled control.