RNA sequencing to detect the differentially expressed genes in AngII infused mice model after QDG treatment.. RNA sequencing to detect the differentially expressed genes in AngII infused mice model after QDG treatment.

The mice (n=15) were randomly divided into 3 groups: Control, Ang Ⅱ, and Ang Ⅱ+QDG groups (n = 5 for each group). Mice in Control and Ang Ⅱ groups were infused with saline or 500 ng/kg/min of Ang Ⅱ respectively, and orally administrated with saline; while mice in Ang Ⅱ + QDG group were infused with Ang Ⅱ (500 ng/kg/min) and orally administrated with 1.145 g/kg /day of QDG for 2 weeks. Then the cardiac tissues were used to identify differentially expressed genes among different groups. Overall design: RNA sequencing was performed with heart tissue of control, AngII and QDG treated (n = 5 for each group ) mice to identify the differently expressed genes and related pathway.