The impact of Spike protein on macrophage inflammation
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Purpose: To elucidate how SARS-CoV-2 spike protein affects macrophage inflammatory responses Methods: Peritoneal macrophages were isolated from Myeloid-specific IKKÃ knockout mice (IKKÃ?Mye) and control littermates (IKKÃ F/F). Then the macrophages were treated with lentivirus expressing Spike protein for 24 hours Results: Deficiency of IKKÃ nearly abolished spike protein-associated geneset scores and DEGs-associated key inflammatory pathways. Conclusions: These results indicate that SARS-CoV-2 Spike protein induces strong inflammatory responses in macrophages, potentially through activation of canonical IKKÃ/NF-?B signaling Overall design: Macrophagic mRNA profiles of control and IKKÃ-deficient macrophages after 24-hour treatment of Lentivirus expressing Spike protein
