Transcriptome analysis in chemical-compound induced brown adipocytes (ciBAs). Homo sapiens

Brown adipogenesis contributes to controlling systemic energy balance by enhancing glucose and lipid consumptions. We have reported chemical compound-induced brown adipocytes (ciBAs) directly converted from human dermal fibroblasts using a serum-free medium. Genome-wide transcriptional analysis was performed in ciBAs in comparison with the control fibroblasts. A broad range of integrated gene expression was enhanced in functional groups including tricarboxylic acid cycle, electron transfer chain, triglycerides metabolism, fatty acid and glucose metabolism, and adaptive thermogenesis. The results suggested that the chemical conversion underwent metabolic and mitochondrial reprogramming closely associated with functions in brown/beige adipocytes. ciBAs could be a promising model for human beige adipocytes applicable for basic research, drug development, and clinical uses.