Differential gene expression analysis of pbl19/pPBL19C3A-GFP-HA transgenic plants

Purpose: RNA-seq analysis of the pbl19/pPBL19:PBL19C3A-GFP-HA, sid2pbl19/pPBL19:PBL19C3A-GFP-HA and eds1pbl19/pPBL19:PBL19C3A-GFP-HA plants and identified a large number of differentially expressed genes in these plants relative to wild-type plants. Methods: For RNA-seq analyses, a total amount of 1 mg RNA per sample was used for RNA-seq library construction. Results: RNA-seq analysis of wild type(WT), pbl19/pPBL19:PBL19C3A-GFP-HA, sid2pbl19/pPBL19:PBL19C3A-GFP-HA and eds1pbl19/pPBL19:PBL19C3A-GFP-HA at 28 DAG.Numbers of differentially expressed genes in pbl19/pPBL19::PBL19C3A-GFP-HA plants compared to WT.1981 differentially expressed genes were constantly up-regulated in pbl19/pPBL19::PBL19C3A-GFP-HA plants compared to WT Conclusions: Receptor-like cytoplasmic kinase PBL19 exhibited the highest transcriptional upregulation among the forty-six genes of the RLCK VII subfamily in pbl19/pPBL19C3A-GFP-HA and sid2 pbl19/pPBL19C3A-GFP-HA transgenic plants.eds1 pbl19/pPBL19:PBL19C3A-GFP-HA plants were fully restored to a wild-type(WT) appearance Overall design: mRNA of 28-day old wild type (WT) and pbl19/pPBL19C3A-GFP-HA, sid2pbl19/pPBL19:PBL19C3A-GFP-HA and eds1pbl19/pPBL19:PBL19C3A-GFP-HA transgenic plants, total 8 samples were analyzed, one biological replicate were conducted in this experiment. WT as a control.