Gene expression signatures for Nanog-positive and Nanog-negative hepatocellular carcinoma cells

Whole genome microarray expression profiling of HCC cell line (Huh7) and patients derive primary HCC cells (T1115 and T1224) expressing Nanog or not. Results provide insight into the role of Nanog in transcriptional regulation. Using Nanog promoter as a reporter system, we sorted the Nanog-positive and Nanog-negative cells from Huh7, T1115 and T1224 by FACS, respectively. Each group is composed of a Nanog negative control and two independent Nanog positive samples.