Upregulated proteins in N. gonorrhoeae biofilms listed by functional rolesa: Biofilm/Planktonic ratios expressed as log2 values, 2-fold increase (log2 values ≥1.000) measured in at least one Extract, and p-values <0.05.

aFunctional role assignments (both main and JCVI sub-roles) for the N. gonorrhoeae proteins were downloaded from the JCVI-CMR website.bAverage log2 values for biofilm/planktonic protein ratios.c(N) represents the number of measurements (biological replicates) averaged.dProteins with more than one functional role assignment provided (Main role/JCVI sub-role): NGO0094, Cellular processes/DNA transformation, Cellular processes/Pathogenesis; NGO1812, Energy metabolism/Fermentation; NGO0718, Energy metabolism/Sugars.eProtein functional role category tentatively assigned in the present study by orthology to an N. meningitidis protein (see Supplementary Table S5 for details).