Five SaCas9 orthologs selected from the NCBI database.
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Five SaCas9 orthologs selected from the NCBI database.
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2022-11-30
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CAS9MODIFICADA MOLECULE ALONE， CAS9MODIFICADA， METHOD FOR PRODUCING A PROTEIN OR RIBONUCLEOPROTE??NA COMPLEXES OR COMPLEXES OF PROTEIN and RIBONUCLEOPROTE??NA MIXED OR LIP??DEO， FUSION PROTEIN， NUCLEOTIDE SEQUENCE， NUCLEIC ACID， VECTOR， CELL， ANIMAL OR PLANT， PHARMACEUTICAL COMPOSITION， IN VITRO USE OF A RECOMBINANT CAS9MODIFICADA， KIT OF PARTS， and IN VITRO METHOD FOR ALTERING THE GENOME OF A CELL
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Additional file 3: of NmeCas9 is an intrinsically high-fidelity genome-editing platform
Table S2. Summary of potential off-target sites detected by GUIDE-seq analysis using the Bioconductor package GUIDE-seq. The table includes sites for both NmeCas9 (with N4GN3 PAM sequences) and SpyCas
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Developing small Cas9 hybrids using molecular modeling
The RNA-guided CRISPR-Cas9 from Streptococcus pyogenes is the best characterized enzyme for gene editing. Its large size, however, precludes it from being packaged together with its single guide (sg)R
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Additional file 14: of CRISPR library designer (CLD): software for multispecies design of single guide RNA libraries
Table S11. Sequences of primers and oligos used for cloning of sgRNA libraries and sequencing. (XLSX 9 kb)
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Straightforward Delivery of Linearized Double-stranded DNA Encoding sgRNA and Donor DNA for the Generation of Single Nucleotide Variants Based on the CRISPR/Cas9 System
CRISPR/Cas9 for genome editing requires delivery of a guide RNA sequence and donor DNA for targeted homologous recombination. Typically, single-stranded oligodeoxynucleotide, serving as the donor temp
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