Nucleotide and amino acid substitution rates in control and treatment groups a.

aNucleotide and amino acid sequences used in the study were compared against Con1 sequence.bNumber of sequences analyzed in each group.cNt. substitution rate is reported as nt. change per site, which was determined as the number of nt. substitutions divided by total number of nucleotides sequenced in each group. Data were normalized by the T7 baseline value which was 1.27×10−3 nt. change per site.dAmino acid substitution rate is reported as amino acid change per site, which was determined by computing the number of amino acid substitutions divided by total number of amino acids analyzed in each group. Data were normalized by the T7 baseline which was 1.96×10−3 amino acid change per site. Note the total number of amino acids analyzed is 1/3 of total number of nucleotides sequenced in each group in c.eStatistically significant difference from control.