RNAseq to profile transcriptomes in bone marrow-derived macrophages from TKO (mir-106a~363-/- mir-106b~25-/- mir-17~92flox/flox Lyz2-Cre) and WT(Lyz2-Cre) mice stimulated with LPS for 3 hours.

We performed the transcriptomic analysis of RNA-seq of BMDMs generated from WT and TKO mice and stimulated with 10 ng/ml LPS for 3 h to identify gene expression changes. BMDMs from WT and TKO mice were stimulated with 10 ng/ml LPS for 3h and then were lysed. RNA was extracted, polyA enriched and prepared for sequencing libraries.