The use of low quality RNA samples in whole-genome gene expression profiling remains controversial. It is unclear if transcript degradation in low quality RNA samples occurs uniformly, in which case t
Background Whole blood expression profiling is frequently performed using PAXgene (Qiagen) or Tempus (Life Technologies) tubes. Here, we compare 6 novel generation RNA isolation protocols with respect
Purpose: The aim of this study is to compare different RNA extraction methods using a mixture design that allows the relative changes of the majority of genes profiled to be estimated. A number of sam
Details of all RNA parameters for both Flash Frozen (FF) and Optimal Cutting Temperature (OCT) processed samples at different time points which include—RIN values, concentration (ng/ul), yield (ug) an