Table 2_Genome-wide identification of m6A methyltransferase genes and m6A modification participates in the response to cold stress in rice.docx

RNA modification is crucial for the post-transcriptional regulatory mechanism that plays a pivotal role in determining RNA structure and function. Among these, N6-methyladenosine (m6A) represents the most abundant one in eukaryotic mRNA. In plants, m6A modification is catalyzed by a complex comprising multiple methyltransferase components. In this study, bioinformatic analyses were employed to characterize the genes of m6A methyltransferases (m6A writers), including their physicochemical properties, structures, cis-acting elements, chromosomal distributions, phylogenetic relationships, and predicted protein structures. Moreover, qRT-PCR and LC-MS/MS were utilized to investigate the expression patterns of m6A writer genes as well as the m6A abundance in total RNA from rice seedlings under low-temperature conditions. Additionally, m6A me-RIP sequencing was performed to explore changes in the m6A profile of mRNA in rice under cold stress. Collectively, our findings revealed the involvement in the regulation of mRNA m6A modification under cold stress in rice.