Molecular correlates of vaccine-induced protection against typhoid fever

Typhoid fever is caused by the Gram-negative bacterium Salmonella enterica serovar Typhi. Bulk RNA-sequencing (RNA-seq) data were generated from blood samples obtained from adult human volunteers enrolled in a vaccine trial involving two vaccines against typhoid fever, a plain polysaccharide vaccine, ViPS and a glycoconjugate vaccine, ViTCV. The participants were then challenged with S. Typhi in a controlled human infection model (CHIM). Whole blood RNA sequencing was performed using paired-end RNA sequencing. Blood samples were taken at several time points post-vaccination and post-challenge to monitor the changes in molecular signatures.