EdU Optimization - Incubation Test #2

Flow cytometry data on mouse fecal bacteria incubated for various lengths of time with (or without) 20 uM 5-ethnyl-2'-deoxyuridine (EdU), a thymidine analogue, to capture the actively replicating bacteria. The bacterial cells underwent a click reaction with Alexa Fluor 647 azide (APC channel) to make newly replicated bacteria fluoresece. They were also stained with Sybr Green I nucleic acid stain (FITC channel) to capture all bacteria.