Transcriptomic analysis provides insight into the mechanism of IKKbeta-mediated suppression of HPV18E6-induced cellular abnormalities

This study is based on a Drosophila model of HPV18E6 plus human E3 ubiquitin ligase (hUBE3A) that we developed recently. Subsequently, taking advantage of the benefits of the fly model, a whole-genome genetic screen revealed that the reduced level and activity of IKKbeta, a regulator of NF-kappa B, suppresses the cellular abnormalities induced by E6 oncoprotein and that the interaction of IKKbeta and E6 is conserved in human cells. In this study, we performed transcriptomic analysis to identify differentially expressed genes that play a role in IKKbeta-mediated suppression of E6-induced defects.