Development of miniBac-seq to profile bacterial transcriptome from ultra-low input RNA amount

We developed miniBac-seq, a strand-specific method for high-quality bacterial RNA-seq library construction from sub-nanogram of total RNA, equivalent to a few thousand bacterial cells; we also tested its potent to be adopted as a single cell RNA-seq method. Here we provide the supporting data to the paper introducing this technology. We used E. coli K12 BW25113, typically cultivated in M63B1 broth and extracted total RNA at the Log phase. Fig2 Impact of customized rRNA depletion method on transcript quantification; Fig4 Overall performance of miniBac-seq; Fig 5 Detection limit of miniBac-seq, via library construction from a gradient of input RNA (5 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg); Fig 6 Impact of barcode introduced at RT on transcript quantification