Motility analysis of wild-type and Tas1r1-deficent sperm.

Computer-assisted sperm analysis (CASA) was performed using an IVOS sperm analyzer (Hamilton Thorne, Berverly, USA). Parameters analyzed are given on the left. Motility values of wild-type [+/+] and Tas1r1 heterozygous [+/−] and homozygous [−/−] sperm are shown as mean values ± SEM of 3 littermate animals for each genotype. Additionally, p-values of a paired Student's T-Test [p values] are given. The following parameters are shown: Percentage of motile sperm [Mot], percentage of sperm with active motility [Prog], averaged path velocity [VAP], straight line velocity [VSL], curvilinear velocity [VCL], amplitude of lateral head displacement [ALH], beat cross frequency [BCF], straightness [STR], linearity [LIN]. A minimum of 2000 spermatozoa was analyzed per animal. Note that wild-type sperm and Tas1r1-deficient spermatozoa did not show any significant differences (p-value≤0.05) in the analyzed motility parameters.