Human Protein Atlas - Subcellular

The subcellular section of the Human Protein Atlas provides high-resolution insights into the expression and spatiotemporal distribution of proteins encoded by 13105 genes (65% of the human protein-coding genes). For each gene, the subcellular distribution of the protein has been investigated by immunofluorescence (ICC-IF) and confocal microscopy in up to three different cell lines, selected from a subset of 37 of the cell lines found in the cell line section. Upon image analysis, the subcellular localization of the protein has been classified into one or more of 35 different organelles and fine subcellular structures. In addition, the section includes an annotation of genes that display single-cell variation in protein expression levels and/or subcellular distribution, as well as an extended analysis of cell cycle dependency of such variations.
The subcellular section offers a database for detailed exploration of individual genes and proteins of interest, as well as for systematic analysis of proteomes in a broader context. More information about the content of the section, as well as the generation and analysis of the data, can be found in the Methods summary.
Learn about:

The subcellular distribution of proteins in human cell lines.
The proteomes of different organelles and subcellular structures.
Single-cell variability in the expression levels and/or localizations of proteins.

该人类蛋白质图谱的亚细胞部分提供了对由13105个基因（占人类蛋白质编码基因的65%）编码的蛋白质表达及其时空分布的高分辨率见解。对于每个基因，蛋白质的亚细胞分布通过免疫荧光（ICC-IF）和共聚焦显微镜在最多三个不同的细胞系中进行了研究，这些细胞系是从细胞系部分中发现的37个细胞系子集中选择出来的。经过图像分析，蛋白质的亚细胞定位被归类为一或多个35种不同的细胞器和精细的亚细胞结构。此外，该部分还包括了对在蛋白质表达水平及/或亚细胞分布上呈现单细胞变异的基因的注释，以及对这种变异的细胞周期依赖性的扩展分析。亚细胞部分提供了一个数据库，用于详细探索感兴趣的个体基因和蛋白质，以及在大范围内对蛋白质组进行系统分析。关于该部分内容的详细信息，以及数据的生成和分析，可在方法总结中找到。了解以下内容：
人类细胞系中蛋白质的亚细胞分布。
不同细胞器和亚细胞结构的蛋白质组。
蛋白质表达水平及/或定位的单细胞变异。