RNA-seq analysis of Mature (EMM) vs. Immature (baseline) iPSC-aCM compared to human atrial tissue (HAT) obtained from the same patient.

The goal of this experiment is to identify gene ontology pathways and differentially expressed genes that distinguish mature iPSC-aCM, immature iPSC-aCM, and human atrial tissue derived from the same patient. Examination of 3 different iPSC-aCM lines at 4 different time points and 2 different culture conditions, as well as 2 different human atrial tissue samples. The first iPSC-aCM line (P1) and the second iPSC-aCM line (P2) are compared at baseline conditions vs. EMM conditions, and compared against human atrial tissue (P1-HAT and P2-HAT) from the same patient. The third iPSC-aCM line (L3) are compared at day 15 (3 samples), day 25 (3 samples), day 32 baseline (3 samples), and day 32 EMM (3 samples).