Human SOD1 overexpressed in motoneurons confers resistance to BMAA treatment.

Shown are survival rates of 5 day-old male sod1 transgenic flies under 3 mM BMAA treatment (10 flies per vial). Panels A, B, and C represent the survival rate of flies expressing mutant human A4V, G85R, wild type SOD1 proteins and control flies (D42>CS and D42>UAS-dsod1) in motoneurons using the D42-Gal4 driver. Flies expressing the human SOD1s (both the hSOD1WT, and mutants, hSOD1A4V and hSOD1G85R) survived longer compared to the control flies. At the 50% survival rate, the longevity is increased by 33%, 41% and 66 % for A4V, G85R, wt SOD1, respectively, when compared to D42>dsod1 and D42>CS flies. Differences were considered statistically significant if pCS and D42>A4V, respectively; n=50 for the rest of genotypes.

本实验展示了经3 mM BMAA处理后的5日龄雄性sod1转基因果蝇的存活率（每管10只果蝇）。图A、B和C分别展示了使用D42-Gal4驱动子，在运动神经元中表达突变型人源A4V、G85R、野生型SOD1蛋白以及对照组果蝇（D42>CS和D42>UAS-dsod1）的存活率。与对照果蝇相比，表达人源SOD1蛋白的果蝇（包括hSOD1WT、突变型hSOD1A4V和hSOD1G85R）的存活时间更长。在50%存活率时，与D42>dsod1和D42>CS果蝇相比，A4V、G85R、野生型SOD1的存活时间分别增加了33%、41%和66%。若差异具有统计学意义，则p值分别为pCS和D42>A4V；其余基因型的样本量均为n=50。