Verification of deletion of the MR from BMDCs. Western blot for the MR using an antibody raised to the MR N terminus. A band was detected in the wild type sample of the correct size for MR (Lanes 1 an
The supplementary data includes confirmatory experiments and reagents performed and generated towards completion of the study. There are three supplementary figures with the corresponding legends, alo
To verify whether the CcpA gene was successfully deleted, we designed the primer for PCR. The primer was designed across the upstream and downstream sequences of the CcpA gene, with a size of about 18