St Johns Island Resistome project

In order to determine if quasi-metagenomics is a potential tool to reveal microbiome metagenomics (and close genome) in comparison with tedious workflow of isolating individual colonies growing on the media and to determine which types of media (agar/broth) will be the best for diversity recovery; water, sediment and seaweed samples were collected from St. John's Island, Singapore. Samples were filtered on 0.22 um membrane and incubated on various types of agar as well as broth and grow at 37oC aerobically. All colonies grew on the agar were scraped using 1.5mL BHI broth and 0.5mL of the aliquot was used for DNA extraction, by Qiagen Power Faecal Pro kit.