An isobaric labeling approach to enhance detection and quan-tification of tissue-derived plasma proteins as potential early disease biomarkers

Accurate detection and quantification of low abundance proteins by standard mass spectrometry approaches remains challenging. In addition, it is difficult to link low abundance plasma proteins back to their specific tissues or organs of origin with confidence. To address these challenges, we developed a mass spectrometry approach based on the use of tandem mass tags (TMT) and a tis-sue reference sample.