Gene expression profiling (RNA-seq) of human iPSCs, iPSC-derived ventralized cells, neurons, and hypothalamic neurons where regions of the genome have been deleted that contain the single nucleotide polymorphisms rs9972768 or rs2650492. Gene expression profiling (RNA-seq) of human iPSCs, iPSC-derived ventralized cells, neurons, and hypothalamic neurons where regions of the genome have been deleted that contain the single nucleotide polymorphisms rs9972768 or rs2650492

Three iPSC lines were differentiated into hypothalamic neuron precursors. Line 1 had an enhancer containing rs9972768 deleted, line 2 had an enhancer containing rs2650492 deleted, and the third line was wild-type. Cells were collected for RNA-seq at 4 time points, 1) iPSCs 2) Ventralized cells 3) Neurons of any type and finally 4) Hypothalamic Neurons. There are either 3 or 4 biological clones represented for each line.