rCRUX Generated Kelly Metazoan 16S Reference Database

rCRUX generated reference database using NCBI nt blast database downloaded in December 2022. Primer Name: Kelly Metazoan 16S<br> Gene: 16S<br> Length of Target: 114-160<br> get_seeds_local() minimum length: 80<br> get_seeds_local() maximum length: 195<br> blast_seeds() minimum length: 50<br> blast_seeds() maximum length: 168<br> max_to_blast: 100<br> Forward Sequence (5'-3'): AGTTACYYTAGGGATAACAGCG<br> Reverse Sequence (5'-3'): CCGGTCTGAACTCAGATCAYGT<br> Reference: Kelly, R. P., O’Donnell, J. L., Lowell, N. C., Shelton, A. O., Samhouri, J. F., Hennessey, S. M., ... &amp; Williams, G. D. (2016). Genetic signatures of ecological diversity along an urbanization gradient. PeerJ, 4, e2444. https://doi.org/10.7717/peerj.2444 We chose default rCRUX parameters for <em>get_blast_seeds</em>() of percent coverage of 70, percent identity of 70, evalue 3e+7, and max number of blast alignments = '100000000' and for <em>blast_seeds</em>() of coverage of 70, percent identity of 70, evalue 3e+7, rank of genus, and max number of blast alignments = '10000000'.