The Human Circular RNA CircHMGCS1 Sponges miR-4521 to Aggravates Diabetes-Induced Vascular Endothelial Dysfunction Through Upregulating ARG1

Cardiovascular diseases pose a significant global health burden. Our study investigates the pivotal role of circular RNAs (circRNAs) and MicroRNAs (miRNAs) in diabetes-induced endothelial dysfunction, a critical aspect of cardiovascular diseases. CircHMGCS1 (hsa_circ_0008621) emerges as a specific circRNA that effectively targets miR-4521. The upregulation of CircHMGCS1 acts as a miR-4521 sponge, facilitating the expression of arginase 1 and accelerating the progression of diabetes-induced endothelial dysfunction. Furthermore, intricate associations among circRNA, miRNA, and mRNA are revealed, illuminating the underlying molecular mechanisms involved in diabetes-induced vascular endothelial dysfunction. These findings significantly advance our understanding of cardiovascular diseases, providing potential therapeutic targets for intervention. In order to simulate the regulatory effect of diabetes on non-coding RNA in vascular endothelium in vitro, We used Human Umbilical Vein Endothelial Cells (HUVECs) to culture them in high fat/high glucose (PAHG) medium for 24h, and The medium contained 25 mM glucose and 250 μM saturated free fatty acid (FFA) palmitate were repeated three times, followed by total RNA extraction for RNA-SEQ analysis